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KMID : 0363620180390010022
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Journal of Korean Oriental Medicine
2018 Volume.39 No. 1 p.22 ~ p.34
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Comparative study on the contents of marker compounds and anti-inflammatory effects of Gamisoyo-san decoction according to storage temperature and periods
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Jin Seong-Eun
Seo Chang-Seob
Lee Na-Ri
Shin Hyeun-Kyoo
Ha Hye-Kyung
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Abstract
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Objectives: The purpose of this study is to investigate changes of the marker compounds and anti-inflammatory effect of Gamisoyo-san decoction (GMSYS) depending on storage temperature and periods.
Methods: GMSYS was stored at room temperature or refrigeration for 12 months. According to storage temperature and periods, pH and sugar content of GMSYS were measured. To determine the marker compounds of GMSYS, high-performance liquid chromatography analysis was performed. To estimate the anti-inflammatory effect of GMSYS, LPS-induced pro-inflammatory mediators and cytokines were measured in RAW 264.7 cells.
Results: There was no change in pH and sugar content depending on storage temperature and periods of GMSYS. The contents of gallic acid and mangiferin in both of room temperature and refrigerated decoctions reduced with increasing storage periods. Chlorogenic acid was time-dependently decreased in case of stored at room temperature. GMSYS significantly inhibited the LPS-induced production of nitric oxide, prostaglandin E2 (PGE2) and IL-6 in RAW 264.7 cells. These effects equally maintained up to 3 months at both of room temperature and refrigeration. Since 4 months, the inhibitory effect of GMSYS on LPS-induced PGE2 production was time-dependently reduced, and the decrease in PGE2 inhibitory effect of decoction stored at refrigeration was lower than that of stored at room temperature.
Conclusions: Our results indicate that the anti-inflammatory effect of GMSYS are maintained up to 12 months, but it shows optimal efficacy up to 3 months. It is recommended to store in a refrigeration for short periods since some components decrease as storage periods becomes longer.
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KEYWORD
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Gamisoyo-san decoction, storage temperature, storage period, high performance liquid chromatography, anti-inflammation
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